Authors: Dominguez, CM; Ramos, D; Mingorance, J; Fierro, JLG; Tamayo, J; Calleja, M
Article.
Anal. Chem.. vol: 90. page: 0003-2700.
Date: JAN 2. 2018.
Doi: 10.1021/acs.analchem.7b04094.
Abstract:
Carbapenem-resistant Enterobacteriaceae have recently become an important cause of morbidity and mortality due to healthcare-associated infections. Most commonly used diagnostic methods are incompatible with fast and accurate directed therapy. We report here the direct identification of the bla(OXA48) gene, which codes for the carbapenemase OXA-48, in lysate samples from Klebsiella pneumoniae. The method is PCR-free and label-free. It is based on the measurement of changes in the stiffness of DNA self-assembled monolayers anchored to microcantilevers that occur as a consequence of the hybridization. The stiffness of the DNA layer is measured through changes of the sensor resonance frequency upon hybridization and at varying relative humidity..
